| Specifications | |
|---|---|
| Dye content (gravimetrically) | ≥ 85 % |
| Identity - sodium |
passes test |
| - UV/VIS-Spectrum | passes test |
| Absorption maximum λ max (water) | 517 - 525 nm |
| Spec. Absorptivity A 1%/1cm (λmax; 0.005 g/l; water) | 800 - 1000 |
| TLC-Test | passes test |
| Loss on drying (110 °C) | ≤ 8 % |
| Suitability for microscopy | passes test |
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Key Spec Table
| CAS # | EC Number | Hill Formula | Molar Mass |
|---|---|---|---|
| 548-24-3 | 208-943-1 | C₂₀H₆Br₂N₂Na₂O₉ | 624.06 g/mol |
| Description | |
|---|---|
| Catalogue Number | 115934 |
| Synonyms | Dibromodinitrofluorescein sodium |
| Description | EOSIN B (C.I. 45400) FOR MICROSCOPY CERT |
| Overview | Eosin B (C.I. 45400) is a CERTISTAIN® dry dye for microscopy that is often used as a counterstain, vizualising proteins, connective tissues, fibers and keratin in sample material of human origin. Depending on user requirements and personal preferences, it can be used for the preparation of the commonly used aqueous or alcoholic Eosin B staining solutions. This staining solution will mostly be used for routine staining in the hematoxylin-eosin staining process (H&E). The H&E staining process is the most frequently used staining method for histological material. Due to the hematoxylin solution, the nuclei will appear blue to dark violet in color and the Eosin Y solution will stain the cytoplasma and intercellular substances red-orange. As the dye belongs to the portfolio of Certistain® dyes, it is chemically analyzed according to strict specifications before release (CE marked and IVD product) and a high staining quality, with reliable staining results is guaranteed. |
| Product Information | |
|---|---|
| CAS number | 548-24-3 |
| EC number | 208-943-1 |
| Hill Formula | C₂₀H₆Br₂N₂Na₂O₉ |
| Molar Mass | 624.06 g/mol |
| HS Code | 3204 12 00 |
| Structure formula Image | |
| Applications | |
|---|---|
| Application | Eosin B (bluish) (C.I.45400) for microscopy Certistain®. CAS 548-24-3, pH 6.2 (10 g/l, H₂O, 25 °C). MSDS and product info below. |
| Physicochemical Information | |
|---|---|
| pH value | 6.2 (10 g/l, H₂O, 25 °C) |
| Bulk density | 680 kg/m3 |
| Solubility | 300 g/l |
| Safety Information according to GHS | |
|---|---|
| Storage class | 10 - 13 Other liquids and solids |
| WGK | WGK 2 water endangering |
| Disposal | 3 Relatively unreactive organic reagents should be collected in container A. If halogenated, they should be collected in container B. For solid residues use container C. |
| Storage and Shipping Information | |
|---|---|
| Storage | Store at +5°C to +30°C. |
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