MacCONKEY broth

Specifications
Appearance (clearness)	clear
Appearance (colour)	violet
pH-value (25 °C)	7.1 - 7.5
Typical composition (g/litre): Peptone from Gelatine 20.0; Lactose 10.0; Ox bile, dried 5.0; Bromcresol purple 0.01.
Growth promotion test in accordance with the harmonised method of EP, USP and JP.
Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013))	10 - 100
Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012))	10 - 100
Inoculum on reference medium (Enterobacter cloacae ATCC 13047 (WDCM 00083))	10 - 100
Inoculum on reference medium (Klebsiella pneumoniae ATCC 13883 (WDCM 00097))	10 - 100
Inoculum on reference medium (Proteus mirabilis ATCC 14273)	10 - 100
Inoculum on reference medium (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025))	10 - 100
Inoculum on reference medium (Staphylococcus aureus ATCC 6538 (WDCM 00032))	≥ 100
Growth (Escherichia coli ATCC 25922 (WDCM 00013))	good to very good
Growth (Escherichia coli ATCC 8739 (WDCM 00012))	good to very good
Growth (Enterobacter cloacae ATCC 13047 (WDCM 00083))	good to very good
Growth (Klebsiella pneumoniae ATCC 13883 (WDCM 00097))	good to very good
Growth (Proteus mirabilis ATCC 14273)	good to very good
Growth (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025))	medium to very good
Growth at 42-44 °C (Escherichia coli ATCC 8739 (WDCM 00012))	good to very good
Growth at 42-44 °C (Staphylococcus aureus ATCC 6538 (WDCM 00032))	none
Colour change to yellow (Escherichia coli ATCC 25922 (WDCM 00013))	+
Colour change to yellow (Escherichia coli ATCC 8739 (WDCM 00012))	+
Colour change to yellow (Enterobacter cloacae ATCC 13047 (WDCM 00083))	+
Colour change to yellow (Klebsiella pneumoniae ATCC 13883 (WDCM 00097))	+
Colour change to yellow (Proteus mirabilis ATCC 14273)	-
Colour change to yellow (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025))	-
Gas (Escherichia coli ATCC 25922 (WDCM 00013))	+
Gas (Escherichia coli ATCC 8739 (WDCM 00012))	+
Gas (Enterobacter cloacae ATCC 13047 (WDCM 00083))	+
Gas (Klebsiella pneumoniae ATCC 13883 (WDCM 00097))	+
Gas (Proteus mirabilis ATCC 14273)	-
Gas (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025))	-
Incubation: 48 hrs. at 30-35°C; E.coli ATCC 8739 also 24 hrs. at 42-44°C; S.aureus: 48 hrs. at 42-44°C
The information above is current at this time of publication and is subject to change without notice (except for customers holding a change control agreement with our company). The information/format can be altered at any time and inany way if internal company matters or Standard Regulationsdo say so.